中药成分检测报告(续断)
项目报告
1 样品信息Sample description
收到续断样品xx个,a和b
A total of xx Dipsaci Radix samples, where received by the customer.
2 材料与方法Materials and Methods
2.1 试剂Chemicals
所有的溶剂与试剂均为色谱级,马钱苷酸,马钱苷,当药苷标准品购于xxx
All chemicals and solvent were HPLC. Standard: loganic acid, loganin, chiratin and were purchased from xxx.
2.2 样品处理Sample Preparation
1) 将样品摇匀,称取0.5g左右的样品;
2) 0.5g样品加入25 mL 50% xxx,称量,超声处理(功率100W,频率40kHz)30 min;
3) 放冷,称量,用50% xxx补足减少的重量,摇匀,并经0.45µm滤膜过滤,进样20 µL。
2.3 HPLC分析HPLC Analysis
本实验所用的仪器为Waters高效液相色谱仪,配备二极管阵列检测器。
色谱柱为xxx C18 (250 mm*4.6 mm, 5 μm),流动相A为0.1% xxx溶液,B为乙腈,流速为1.0 mL/min,柱温为25℃。
3 结果Results
色谱图Chromatogram
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