植物色素检测报告(枸杞)

项目报告
1 样品信息Sample description
收到枸杞样品xx个,a和b
A total of xx Lycium chinensis samples, where received by the customer.
2 材料与方法Materials and Methods
2.1 试剂Chemicals
所有的溶剂与试剂均为色谱级,飞燕草色素,矢车菊色素,牵牛花色素,天竺葵色素,芍药色素,锦葵色素标准品购于xxx
All chemicals and solvent were HPLC. Standard: delphinidin, cyanidin, petunidin, pelargonidin, peonidin, malvidin and were purchased from xxx.
2.2 样品处理Sample Preparation
1) 将样品摇匀,称取1.0 g左右的样品;
2) 1.0 g样品加入xxx mL酸化乙醇提取剂,超声提取30 min,沸水浴中冷凝回流1 h;
3) 冷却至室温后,用0.22 µm滤膜过滤,进样10 µL。
2.3 HPLC分析HPLC Analysis
本实验所用的仪器为Waters 2695型高效液相色谱仪,配备紫外检测器。
色谱柱为Zorbax SB-C18 (250 mm*4.6 mm, 5 μm),流动相乙腈/0.1% xxx溶液,流速为xxx mL/min,柱温为40℃。
3 结果Results
色谱图Chromatogram


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