植物色素检测报告(檵木)

项目报告
1 样品信息Sample description
收到檵木样品xx个,a和b
A total of xx loropetalum samples, where received by the customer.
2 材料与方法Materials and Methods
2.1 试剂Chemicals
所有的溶剂与试剂均为色谱级,槲皮素,山奈酚,杨梅素标准品购于xxx
All chemicals and solvent were HPLC. Standard: quercetin , kaempferol and myricetin and were purchased from xxx.
2.2 样品处理Sample Preparation
1) 将样品摇匀,称取1.0g左右的样品;
2) 1.0g样品加入xx%甲醇50 mL,称重,回流提取1h;
3) 放冷,称重,用xx%甲醇补足减失的重量,过滤;
4) 吸取滤液25 mL,加入5% xxx 10 mL、甲醇5 mL,回流30 min;
5) 放冷,移至xx mL量瓶中,用甲醇洗涤3次,一并移至量瓶中,加甲醇至刻度,摇匀并用0.45 µm滤膜过滤,进样10 µL。
2.3 HPLC分析HPLC Analysis
本实验所用的仪器为Agilent高效液相色谱仪,配备二极管阵列检测器。
色谱柱为Thermo xxx C18 (150 mm*4.6 mm, 5 μm),流动相为乙腈-0.4% xxx水溶液(32:68,v/v),流速为1.0 mL/min,柱温为25℃。
3 结果Results
色谱图Chromatogram


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