核苷检测报告(猪肝)

项目报告
1 样品信息Sample description
收到猪肝样品xx个,a和b
A total of xx pork liver samples, where received by the customer.
2 材料与方法Materials and Methods
2.1 试剂Chemicals
所有的溶剂与试剂均为色谱级,鸟嘌呤,次黄嘌呤,黄嘌呤,腺嘌呤标准品购于xxx
All chemicals and solvent were HPLC. Standard: guanine, hypoxanthine, xanthine, adenine and were purchased from xxx.
2.2 样品处理Sample Preparation
1) 将样品摇匀,称取0.2g左右的样品;
2) 0.2g样品加入1 mL超纯水、5 mL xxx、5 mL甲酸混合,90℃水浴振荡12 min;
3) 冷却,转至梨形瓶中,50℃水浴旋转蒸发至干;
4) 残留物加10 mL 0.02 mol/L xxx溶液(pH=3.8)溶解,用0.22 µm滤膜过滤,进样10 µL。
2.3 HPLC分析HPLC Analysis
本实验所用的仪器为Agilent高效液相色谱仪,配备二极管阵列检测器。
色谱柱为xxx C18 (250 mm*4.6 mm, 5 μm),流动相为0.02 mol/L xxx溶液(pH=3.8),流速为1.0 mL/min,柱温为30℃。
3 结果Results
色谱图Chromatogram


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