有机酸检测报告（香菇）

项目报告

1 样品信息Sample description
收到香菇样品xx个，a和b
A total of xx Lentinus edodes samples, where received by the customer.

2 材料与方法Materials and Methods
2.1 试剂Chemicals
所有的溶剂与试剂均为色谱级，酒石酸，苹果酸，乙酸，柠檬酸标准品购于xxx
All chemicals and solvent were HPLC. Standard: tartaric acid, malic acid, acetic acid, citric acid and were purchased from xxx.

2.2 样品处理Sample Preparation
1) 将样品摇匀，称取0.5g左右的样品；
2) 0.5g样品中加入0.1 mol/L xxx溶液，xx℃水浴振荡提取30 min；
3) 冷却，10000 r/min离心10 min，上清液用0.22 µm滤膜过滤，进样10 µL。

2.3 HPLC分析HPLC Analysis
本实验所用的仪器为Waters高效液相色谱仪，配备紫外检测器。
色谱柱为xxx C18 (250 mm*4.6 mm, 5 μm)，流动相为2% xxx，流速为1.0 mL/min，柱温为35℃。

3 结果Results
色谱图Chromatogram