有机酸检测报告(苹果)

项目报告
1 样品信息Sample description
收到苹果样品xx个,a和b
A total of xx apple samples, where received by the customer.
2 材料与方法Materials and Methods
2.1 试剂Chemicals
所有的溶剂与试剂均为色谱级,草酸,酒石酸,苹果酸,柠檬酸,琥珀酸,乙酸标准品购于xxx
All chemicals and solvent were HPLC. Standard: oxalic acid, tartaric acid, malic acid, citric acid, succinic acid, acetic acid and were purchased from xxx.
2.2 样品处理Sample Preparation
1) 将样品摇匀,称取5.0g样品;
2) 5.0g样品中加入25 mL xxx,研磨成匀浆,80℃水浴超声提取1h;
3) 冷却,10000 r/min离心15 min,收集上清液,残渣加入15 mL xxx重复提取1次;
4) 合并上清液,用xxx定容至50 mL,并经0.45 µm滤膜过滤,进样10 µL。
2.3 HPLC分析HPLC Analysis
本实验所用的仪器为Waters高效液相色谱仪,配备二极管阵列检测器。
色谱柱为xxx C18 (250 mm*4.6 mm, 5 μm),流动相为0.01 mol/L xxx溶液,流速为0.5 mL/min,柱温为50℃。
3 结果Results
色谱图Chromatogram


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