植物多酚检测报告（香蕉皮）

项目报告

1 样品信息Sample description
收到香蕉皮样品xx个，a和b
A total of xx banana peel samples, where received by the customer.

2 材料与方法Materials and Methods
2.1 试剂Chemicals
所有的溶剂与试剂均为色谱级，原儿茶酸，儿茶素，绿原酸，咖啡酸，阿魏酸，芦丁标准品购于Sigma-Aldrch.
All chemicals and solvent were HPLC. Standard: protocatechuate, catechin, chlorogenic acid, caffeic acid, ferulic acid, rutin and were purchased from Sigma-Aldrch.

2.2 样品处理Sample Preparation
1) 将样品摇匀，称取10.0g左右的样品；
2) 10.0g样品加入60% xxx溶液，超声提取（功率250W，温度50℃）15 min；
3) 过滤，滤液用30% xxx溶液定容至25 mL，摇匀，用0.22 µm滤膜过滤，进样5 µL。

2.3 HPLC分析HPLC Analysis
本实验所用的仪器为Agilent 1100型高效液相色谱仪，配备二极管阵列检测器。
色谱柱为xxx-C18 (125 mm*4.6 mm, 5 μm)，流动相A为xxx，B为0.01 mol/L xxx溶液，流速为0.8 mL/min，柱温为40℃。

3 结果Results
色谱图Chromatogram