植物多酚检测报告(石榴皮)

项目报告
1 样品信息Sample description
收到石榴皮样品xx个,a和b
A total of xx pomegranate peel samples, where received by the customer.
2 材料与方法Materials and Methods
2.1 试剂Chemicals
所有的溶剂与试剂均为色谱级,绿原酸,表儿茶素,鞣花酸,槲皮素标准品购于xxx
All chemicals and solvent were HPLC. Standard: chlorogenic acid, L-epicatechin, ellagic acid, quercetin and were purchased from xxx.
2.2 样品处理Sample Preparation
1) 将样品摇匀,称取0.5g左右的样品;
2) 0.5g样品加入50% xxx溶液,超声提取(功率250W,温度50℃)15 min;
3) 过滤,滤液用50% xxx溶液定容至50 mL,摇匀,用0.22 µm滤膜过滤,进样20 µL。
2.3 HPLC分析HPLC Analysis
本实验所用的仪器为Waters高效液相色谱仪,配备紫外检测器。
色谱柱为xxx ODS2 (250 mm*4.6 mm, 5 μm),流动相为乙腈-0.4% xxx溶液,流速为1.0 mL/min,柱温为30℃。
3 结果Results
色谱图Chromatogram


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