糖类检测报告(饼干)
项目报告
1 样品信息Sample description
收到饼干样品xx个,a和b
A total of xx biscuit samples, where received by the customer.
2 材料与方法Materials and Methods
2.1 试剂Chemicals
所有的溶剂与试剂均为色谱级,赤藓糖醇,木糖醇,果糖,山梨糖醇,甘露糖醇,葡萄糖,蔗糖,麦芽糖醇,麦芽糖,乳糖标准品购于xxx
All chemicals and solvent were HPLC. Standard: meso-erythritol, xylitol, fructose, sorbitol, mannitol, glucose, sucrose, maltitol, maltose, lactose and were purchased from xxx.
2.2 样品处理Sample Preparation
1) 将样品摇匀,称取2.0g左右样品;
2) 2.0g样品中加入50 mL蒸馏水,超声提取30 min;
3) 缓慢加入5 mL xxx和5 mL xxx溶液,转移至100 mL容量瓶,加水定容至刻度,混匀,5000 r/min离心5 min;
4) 取上清液用0.45 µm滤膜过滤,进样10 µL。
2.3 HPLC分析HPLC Analysis
本实验所用的仪器为Waters高效液相色谱仪,配备蒸发光散射检测器。
色谱柱为xxx Carbohydrate ES (250 mm*4.6 mm, 5 μm),流动相为xxx-水,流速为1.0 mL/min,柱温为30℃。
3 结果Results
色谱图Chromatogram